When urea is heated,it forms biuret,the alkal | vedclass

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(A) When urea $(NH_2CONH_2)$ is heated,two molecules of urea condense to form biuret $(NH_2CONHCONH_2)$ with the elimination of an ammonia molecule $(

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Violet colour

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(A) When urea $(NH_2CONH_2)$ is heated,two molecules of urea condense to form biuret $(NH_2CONHCONH_2)$ with the elimination of an ammonia molecule $(NH_3)$.In the presence of an alkaline solution (usually $NaOH$),biuret reacts with copper$(II)$ sulfate $(CuSO_4)$ to form a complex compound.This reaction is known as the biuret test,which produces a characteristic $Violet$ colour,indicating the presence of peptide bonds.

List-$I$ (Amino acid)	List-$II$ (Positive reaction/Test for functional group present in side chain of amino acid)
$A$. Glutamine	$I$. Hinsberg's test
$B$. Lysine	$II$. Neutral $FeCl_3$ test
$C$. Tyrosine	$III$. Ceric ammonium nitrate test
$D$. Serine	$IV$. Hoffman bromamide degradation

When urea is heated,it forms biuret,the alkaline solution of which forms $.....$ with $CuSO_4$ solution.

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