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(C) In recombinant $DNA$ technology,vector $DNA$ molecules can undergo self-ligation if their $5'$ ends possess phosphate groups. Alkaline phosphatase

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Alkaline phosphatase

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(C) In recombinant $DNA$ technology,vector $DNA$ molecules can undergo self-ligation if their $5'$ ends possess phosphate groups. Alkaline phosphatase is an enzyme that removes the $5'$ phosphate groups from the $DNA$ molecules. By removing these phosphate groups,the enzyme prevents the vector from re-annealing or self-ligating,thereby ensuring that only the desired insert $DNA$ can be ligated into the vector.

Which enzyme is required to prevent unwanted self-ligation of vector $DNA$ molecules in recombinant $DNA$ technology?

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