Which of the following is correct for polymerase chain reaction $(\text{PCR})$?

  • A
    In $\text{PCR}$ multiple copies of $\text{DNA}$ are synthesized in the cell.
  • B
    The correct order of steps is denaturation,extension,and annealing,respectively.
  • C
    The $\text{DNA}$ probe used is complementary to the $5'$ end of the template strand.
  • D
    The $\text{DNA}$ polymerase used in $\text{PCR}$ is thermostable and remains active during high temperatures.

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Similar Questions

Identify the correct sequence of steps in the Polymerase Chain Reaction $(PCR)$.

Given below are four statements pertaining to the separation of $DNA$ fragments using gel electrophoresis. Identify the incorrect statements:
$(a)$ $DNA$ is a negatively charged molecule and so it is loaded on the gel towards the Anode terminal.
$(b)$ $DNA$ fragments travel along the surface of the gel,whose concentration does not affect the movement of $DNA$.
$(c)$ The smaller the size of the $DNA$ fragment,the larger is the distance it travels through it.
$(d)$ Pure $DNA$ can be visualized directly by exposing it to $UV$ radiation.
Choose the correct answer from the options given below:

At which stage of $PCR$,does the template join with the primer?

Having become an expert on gel electrophoresis,you are asked to examine a gel for a colleague. Where would you find the smallest segment of $DNA$?

Which of the following range of temperature is required for denaturation,annealing of primer,and primer extension of $PCR$ respectively?

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