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(B) In aerobic respiration,glycolysis is linked to the Krebs' cycle through the formation of acetyl Co-$A$. This process,known as the link reaction or

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Vitamin-$B_{1}$

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(B) In aerobic respiration,glycolysis is linked to the Krebs' cycle through the formation of acetyl Co-$A$. This process,known as the link reaction or oxidative decarboxylation of pyruvic acid,is catalyzed by the pyruvate dehydrogenase complex. This enzyme complex requires several cofactors for its activity,including $Mg^{2+}$,thiamine pyrophosphate (Vitamin-$B_{1}$),$NAD^{+}$,Co-$A$,and lipoic acid. Therefore,Vitamin-$B_{1}$ is essential for the catalysis of the link reaction.

Respiration	Type of substrate
$A$. Floating respiration	$i$. Proteins
$B$. Cytoplasmic respiration	$ii$. Starch
$C$. Protoplasmic respiration	$iii$. Carbohydrates
$D$. Lactic acid fermentation	$iv$. Lactose

Which of the following is involved in the catalysis of link reaction during aerobic respiration?

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