Which of the following may be considered as an application of biotechnology?

Read the following four statements and identify the two incorrect statements:
$(1)$ The first transgenic buffalo,Rosie,produced milk enriched with human alpha-lactalbumin.
$(2)$ $DNA$ is isolated from large molecules using restriction enzymes.
$(3)$ Downstream processing is a stage of $r$-$DNA$ technology.
$(4)$ Disarmed pathogen vectors are also used to transfer $r$-$DNA$ into the host.

Match the columns:

Column-$I$	Column-$II$
$(1)$ Golden Rice	$(a)$ Bud insects
$(2)$ Bt toxin	$(b)$ Vitamin-$A$
$(3)$ Biopiracy	$(c)$ Cry-protein
$(4)$ Bt tobacco	$(d)$ Bio-source without permission and use of bioreactor

Product of biotechnology is

Explain the use of biotechnology in various fields.

Statement $I$: $\text{RNA}$ interference $\text{(RNAi)}$ takes place in all eukaryotic organisms as a method of cellular defense.
Statement $II$: $Bt$ toxin protein is converted into an active form of toxin due to the alkaline $pH$ of the gut of an insect.