Which of the following steps are involved in the process of recombinant biotechnology? Arrange in correct order.
$I.$ Extraction of the desired gene product
$II.$ Amplification of the gene of interest
$III.$ Isolation of a desired $DNA$ fragment
$IV.$ Ligation of the $DNA$ fragment into a vector
$V.$ Insertion of recombinant $DNA$ into the host
Correct order is:

• A
  $I, II, III, IV, V$
• B
  $III, II, IV, V, I$
• C
  $II, IV, V, III, I$
• D
  $I, IV, V, III, II$