Which one of the following statements is wron | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(D) The correct answer is $D$. The statement 'The smaller $DNA$ fragments separate first' is technically incorrect in the context of the final separat

Vedclass · Accepted Answer

The smaller $DNA$ fragments separate first.

Vedclass · Answer

(D) The correct answer is $D$. The statement 'The smaller $DNA$ fragments separate first' is technically incorrect in the context of the final separation pattern,as they migrate the fastest and reach the farthest point,but they do not 'separate first' in a chronological sense; rather,they are the ones that travel the furthest distance through the gel matrix.Gel electrophoresis is a technique that uses an electric field to separate $DNA$ molecules based on their size.Since $DNA$ molecules are negatively charged due to the phosphate backbone,they migrate towards the positive electrode (anode).The rate of migration is inversely proportional to the size of the $DNA$ fragment.Smaller $DNA$ fragments encounter less resistance from the agarose matrix and thus migrate faster and travel further than larger fragments.Therefore,the band located farthest from the loading well represents the smallest $DNA$ fragment.

Which one of the following statements is wrong with respect to separation of $DNA$ fragments on gel electrophoresis?

Similar Questions

Which of the following statements is correct in the context of observing $DNA$ separated by agarose gel electrophoresis?

The steps involved in the Southern blot test are as follows:
$I.$ $X-ray$ film
$II.$ Electrophoresis
$III.$ Digestion with restriction enzyme
$IV.$ Ethidium bromide
$V.$ Radioactive probe
Choose the option having the correct sequential order of these events.

After $4$ $PCR$ cycles,how many $DNA$ molecules are formed from one $DNA$ template molecule?

What is the first step in the Southern Blotting technique?

Polymerase chain reaction $\text{(PCR)}$ amplifies $\text{DNA}$ following the equation:

Vedclass Test Series

Exam Paper Generator

Online Exam Module