Which one of the following techniques does not serve the purpose of early diagnosis of a disease for its early treatment?

Hybridoma cells are

Identify the correct statement.

When a gene is targeted to correct a disease by amplifying the gene,what is this process called?

Some of the steps involved in the production of humulin are given below. Choose the correct sequence.
$(i)$ Synthesis of gene $(DNA)$ for human insulin artificially.
(ii) Culturing recombinant $E. coli$ in bioreactors.
(iii) Purification of humulin.
(iv) Insertion of human insulin gene into plasmid.
$(v)$ Introduction of recombinant plasmid into $E. coli$.
(vi) Extraction of recombinant gene product from $E. coli$.

Match the following columns:

List-$I$	List-$II$
$(a)$ $1977$	$(1)$ Creation of the first transgenic cow
$(b)$ $1983$	$(2)$ American company obtained patent for Basmati rice
$(c)$ $1990$	$(3)$ Eli Lilly company prepared two $DNA$ sequences for insulin
$(d)$ $1997$	$(4)$ First clinical gene therapy trial