Which steps are included in recombinant $DNA$ technology mechanism?
(N/A) Recombinant $DNA$ technology involves several steps in a specific sequence:
$1$. Isolation of the genetic material $(DNA)$.
$2$. Fragmentation of $DNA$ by restriction endonucleases.
$3$. Isolation of a desired $DNA$ fragment.
$4$. Ligation of the $DNA$ fragment into a vector.
$5$. Transferring the recombinant $DNA$ into the host.
$6$. Culturing the host cells in a medium at a large scale.
$7$. Extraction of the desired product.
$1$. Isolation of the genetic material $(DNA)$.
$2$. Fragmentation of $DNA$ by restriction endonucleases.
$3$. Isolation of a desired $DNA$ fragment.
$4$. Ligation of the $DNA$ fragment into a vector.
$5$. Transferring the recombinant $DNA$ into the host.
$6$. Culturing the host cells in a medium at a large scale.
$7$. Extraction of the desired product.
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Similar Questions
Choose the correct statement:
$P -$ Endonucleases cut the $DNA$ at a specific site inside the $DNA$.
$Q -$ $A$ stirred-tank reactor is usually square in shape.
$R -$ In order to force bacteria to take up the plasmid,the bacterial cells must first be made 'competent' to take up $DNA$ by treating them with a divalent cation like $Ca^{2+}$.
$P -$ Endonucleases cut the $DNA$ at a specific site inside the $DNA$.
$Q -$ $A$ stirred-tank reactor is usually square in shape.
$R -$ In order to force bacteria to take up the plasmid,the bacterial cells must first be made 'competent' to take up $DNA$ by treating them with a divalent cation like $Ca^{2+}$.
In gel electrophoresis,the separated $DNA$ fragments can be visualized by:
What does 'competent' refer to in competent cells used in transformation experiments?
Medium
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$P \quad \quad Q \quad \quad R$
$P \quad \quad Q \quad \quad R$
$mRNA$ silencing is known as .........
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