While isolating DNA from bacteria,which of th | vedclass

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(D) To isolate $DNA$ from bacterial cells,the cell wall must be broken down and other macromolecules like $RNA$ and proteins must be removed.$1$. Lyso

Vedclass · Accepted Answer

Deoxyribonuclease

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(D) To isolate $DNA$ from bacterial cells,the cell wall must be broken down and other macromolecules like $RNA$ and proteins must be removed.$1$. Lysozyme is used to break down the bacterial cell wall.$2$. Ribonuclease is used to degrade $RNA$.$3$. Protease is used to degrade proteins.$4$. Deoxyribonuclease $(DNase)$ is an enzyme that breaks down $DNA$. If $DNase$ is added during the isolation process,it will degrade the $DNA$ we are trying to extract. Therefore,it is not used.

Column-$I$	Column-$II$
$(1)$ Bacterial membrane	$(p)$ Chitinase
$(2)$ Plant cells	$(q)$ Ribonuclease
$(3)$ Fungal membrane	$(r)$ Lysozyme
$(4)$ $RNA$ molecule	$(s)$ Cellulase

While isolating $DNA$ from bacteria,which of the following enzymes is not used?

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Match the following:

Column-$I$ Column-$II$

$(1)$ Bacterial membrane $(p)$ Chitinase

$(2)$ Plant cells $(q)$ Ribonuclease

$(3)$ Fungal membrane $(r)$ Lysozyme

$(4)$ $RNA$ molecule $(s)$ Cellulase

Which antibiotic resistance genes are present in $pBR322$?

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