Would you choose an exonuclease while produci | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(B) No,an exonuclease would not be chosen. Exonucleases act on the free ends of a linear $DNA$ molecule by removing nucleotides one by one. Instead of

Vedclass · Accepted Answer

No,because it degrades the $DNA$ from the ends.

Vedclass · Answer

(B) No,an exonuclease would not be chosen. Exonucleases act on the free ends of a linear $DNA$ molecule by removing nucleotides one by one. Instead of producing $DNA$ fragments with sticky ends suitable for ligation,an exonuclease would shorten or completely degrade the $DNA$ fragment containing the gene of interest. Furthermore,a circular plasmid (vector) lacks free ends,so an exonuclease would not be able to cut it at all. Therefore,restriction endonucleases are preferred as they cut $DNA$ at specific internal sites to produce sticky or blunt ends.

Would you choose an exonuclease while producing a recombinant $DNA$ molecule?

Similar Questions

In the gel electrophoresis method . . . . . . .

Which of these are most widely used in genetic engineering?

Which statement is correct for the $tet^R$ gene in the $pBR322$ plasmid?

The process of replication in plasmid $DNA$,other than initiation,is controlled by

In $pBR322$,what does the letter '$p$' stand for?

Vedclass Test Series

Exam Paper Generator

Online Exam Module