Which of the following is not used in the Sou | vedclass

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(D) Southern hybridization is a technique used for the detection of specific $DNA$ sequences in $DNA$ samples.The steps involved in Southern hybridiza

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$PCR$

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(D) Southern hybridization is a technique used for the detection of specific $DNA$ sequences in $DNA$ samples.The steps involved in Southern hybridization are:$1$. Digestion of $DNA$ with restriction endonucleases.$2$. Separation of $DNA$ fragments by gel electrophoresis.$3$. Transfer of separated $DNA$ fragments onto a nitrocellulose or nylon membrane (Blotting).$4$. Hybridization with a labeled probe.$5$. Detection of the hybridized probe using autoradiography.$PCR$ (Polymerase Chain Reaction) is a separate technique used for the amplification of specific $DNA$ sequences and is not a step in the standard Southern hybridization procedure.

Which of the following is not used in the Southern hybridization technique for chromosomal $DNA$ analysis?

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