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(A) The cloning vector $pBR322$ is a widely used plasmid vector in genetic engineering.To facilitate the cloning process,it is essential that the vect

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(A) The cloning vector $pBR322$ is a widely used plasmid vector in genetic engineering.To facilitate the cloning process,it is essential that the vector contains unique recognition sites for various restriction enzymes.If a restriction enzyme had multiple recognition sites within the vector,the plasmid would be cleaved into multiple fragments upon digestion,which would make the insertion of the foreign $DNA$ segment impossible or highly inefficient.Therefore,$pBR322$ is engineered to have only $1$ (one) unique recognition site for each of the specific restriction enzymes used for cloning,such as $EcoRI$,$BamHI$,$HindIII$,etc.

Column-$I$	Column-$II$
$P$. Restriction endonuclease	$I$. Synthesizes $DNA$ copies
$Q$. $DNA$ polymerase	$II$. Cleaves $DNA$ molecules
$R$. $DNA$ ligase	$III$. Joins $DNA$ molecules

How many recognition sites are present for each restriction enzyme in the cloning vector $pBR322$?

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Plasmids are suitable vectors for gene cloning because...

Match the enzymes in Column-$I$ with their respective functions in Column-$II$.

Column-$I$ Column-$II$

$P$. Restriction endonuclease $I$. Synthesizes $DNA$ copies

$Q$. $DNA$ polymerase $II$. Cleaves $DNA$ molecules

$R$. $DNA$ ligase $III$. Joins $DNA$ molecules

Which restriction enzymes have recognition sites within the antibiotic resistance genes $amp^R$ and $tet^R$ in the plasmid $pBR322$?

Select the option that excludes characteristics applicable to plasmids:
$(a)$. Circular $DNA$
$(b)$. Linear $DNA$
$(c)$. Present in all bacteria
$(d)$. Contain essential genes
$(e)$. Extra chromosomal self-replicating

What do '$H$','$in$','$d$',and '$III$' refer to in the enzyme $\text{Hind III}$?

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