Select the correct sequence for PCR (Polymera | vedclass

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Question

(C) $PCR$ (Polymerase Chain Reaction) involves three main steps in each cycle:$1$. Denaturation: The double-stranded $DNA$ is heated to a high tempera

Vedclass · Accepted Answer

Denaturation $\rightarrow$ Annealing $\rightarrow$ Extension

Vedclass · Answer

(C) $PCR$ (Polymerase Chain Reaction) involves three main steps in each cycle:$1$. Denaturation: The double-stranded $DNA$ is heated to a high temperature (approx. $94-98^{\circ}C$) to separate the strands.$2$. Annealing: The temperature is lowered (approx. $50-65^{\circ}C$) to allow primers to bind to the complementary sequences on the single-stranded $DNA$.$3$. Extension: The temperature is adjusted (approx. $72^{\circ}C$) for the $Taq$ polymerase to synthesize the new $DNA$ strand by adding nucleotides.Therefore,the correct sequence is Denaturation $\rightarrow$ Annealing $\rightarrow$ Extension.

Select the correct sequence for $PCR$ (Polymerase Chain Reaction).

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