A : Taq Polymerase is involved in PCR techniq | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(A) The $PCR$ $(Polymerase Chain Reaction)$ technique requires a thermostable $DNA$ polymerase enzyme to synthesize new $DNA$ strands.$Taq$ Polymerase

Vedclass · Accepted Answer

Assertion and Reason both are correct and Reason is the correct explanation of Assertion.

Vedclass · Answer

(A) The $PCR$ $(Polymerase Chain Reaction)$ technique requires a thermostable $DNA$ polymerase enzyme to synthesize new $DNA$ strands.$Taq$ Polymerase is isolated from the bacterium $Thermus$ $aquaticus$.This enzyme is thermostable,meaning it can withstand the high temperatures (around $94-95^{\circ}C$) required for the denaturation step of $PCR$ without getting denatured itself.Therefore,both the Assertion and the Reason are correct,and the Reason provides the correct explanation for why $Taq$ Polymerase is used in $PCR$.

$A$ : Taq Polymerase is involved in $PCR$ technique.
$R$ : This enzyme remains active during the high temperature including denaturation of double stranded $DNA$.

Similar Questions

By which process is a fragment of $DNA$ transferred into a bacterial host cell?

From the given combination of steps in $PCR$,identify the enzyme-dependent step.

Ethidium bromide stained agarose gel is exposed to $.......,$ to observe $\text{DNA}$ as bright orange coloured bands.

Which one of the following statements is wrong with respect to separation of $DNA$ fragments on gel electrophoresis?

$A$ simple stirred-tank bioreactor is shown below. Identify $A, B, C, D$,and $E$.

Vedclass Test Series

Exam Paper Generator

Online Exam Module

$A$ : Taq Polymerase is involved in $PCR$ technique.$R$ : This enzyme remains active during the high temperature including denaturation of double stranded $DNA$.