In the $PCR$ technique,the amplification of a $DNA$ segment is carried out by which enzyme?

The restriction enzyme responsible for the cleavage of the following sequence is:
$5^{\prime}-G-T-C-G-A-C-3^{\prime}$
$3^{\prime}-C-A-G-C-T-G-5^{\prime}$

$A$ plasmid $DNA$ and a linear $DNA$ (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis,the plasmid shows one $DNA$ band while the linear $DNA$ shows two fragments. Explain.

While isolating $DNA$ from bacteria,which of the following enzymes is not used?

Which of the following is used in recombinant $DNA$ technique?

Which of the following has popularized the $PCR$ (polymerase chain reaction)?