What should be the constant temperature maintained for the inoculum (in $^oC$)?

In $r-DNA$ technology,the term 'elution' refers to:

Consider the following figure of a stirred-tank bioreactor and identify $A, B, C$,and $D$.

Which of the following range of temperature is required for denaturation,annealing of primer,and primer extension of $PCR$ respectively?

Given below are two statements:
Statement $I:$ The $\text{DNA}$ fragments extracted from gel electrophoresis can be used in the construction of recombinant $\text{DNA}$.
Statement $II:$ Smaller size $\text{DNA}$ fragments are observed near the anode while larger fragments are found near the wells in an agarose gel.
In the light of the above statements,choose the most appropriate answer from the options given below:

During the process of gene amplification using $PCR$,if very high temperature is not maintained in the beginning,then which of the following steps of $PCR$ will be affected first?