During the process of gene amplification using $PCR$,if very high temperature is not maintained in the beginning,then which of the following steps of $PCR$ will be affected first?

Which of the following steps are involved in the process of recombinant biotechnology? Arrange in correct order.
$I.$ Extraction of the desired gene product
$II.$ Amplification of the gene of interest
$III.$ Isolation of a desired $DNA$ fragment
$IV.$ Ligation of the $DNA$ fragment into a vector
$V.$ Insertion of recombinant $DNA$ into the host
Correct order is:

The Polymerase Chain Reaction $(PCR)$ is a reaction in which amplification of specific $DNA$ sequences is carried out in vitro. This statement is

What is the process of elution?

Which steps are included in recombinant $DNA$ technology mechanism?

Select the correct statements from the following: