Class 12BiologyBiotechnology: Principles and ProcessesMix Examples - Biotechnology Principals and Process
MediumThe given figure shows the cloning vector $pBR322$. Identify the labels $A, B, C,$ and $D$ correctly.
- A$A \to \text{Hind III}, B \to \text{EcoR I}, C \to \text{amp}^R, D \to \text{ori}$
- B$A \to \text{Hind III}, B \to \text{Bam HI}, C \to \text{kan}^R, D \to \text{amp}^R$
- C$A \to \text{Bam HI}, B \to \text{Pst I}, C \to \text{ori}, D \to \text{amp}^R$
- D$A \to \text{EcoR I}, B \to \text{Bam HI}, C \to \text{amp}^R, D \to \text{ori}$
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Similar Questions
Assertion : In recombinant $DNA$ technology,human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote).
Reason : Both bacteria and yeast multiply very fast to form huge population,which express the desired gene.
Reason : Both bacteria and yeast multiply very fast to form huge population,which express the desired gene.
MediumAIIMS 2015
View SolutionMatch the following techniques or instruments with their usage:
Column-$I$ Column-$II$ $(a)$ Bioreactor $(i)$ Separation of $DNA$ fragments $(b)$ Electrophoresis $(ii)$ Production of large quantities of products $(c)$ $PCR$ $(iii)$ Detection of pathogen, based on antigen-antibody reaction $(d)$ $ELISA$ $(iv)$ Amplification of nucleic acids
Select the correct option from the following:
| Column-$I$ | Column-$II$ |
| $(a)$ Bioreactor | $(i)$ Separation of $DNA$ fragments |
| $(b)$ Electrophoresis | $(ii)$ Production of large quantities of products |
| $(c)$ $PCR$ | $(iii)$ Detection of pathogen, based on antigen-antibody reaction |
| $(d)$ $ELISA$ | $(iv)$ Amplification of nucleic acids |
Select the correct option from the following:
MediumNEET 2020
View SolutionGiven below are two statements: one is labelled as Assertion $(A)$ and the other is labelled as Reason $(R)$.
Assertion $(A):$
Polymerase chain reaction is used in $DNA$ amplification.
Reason $(R):$
The ampicillin resistant gene is used as a selectable marker to check transformation.
In the light of the above statements,choose the correct answer from the options given below:
Assertion $(A):$
Polymerase chain reaction is used in $DNA$ amplification.
Reason $(R):$
The ampicillin resistant gene is used as a selectable marker to check transformation.
In the light of the above statements,choose the correct answer from the options given below:
MediumNEET 2022
View SolutionMatch the organism with its use in biotechnology:
$(a)$ Bacillus thuringiensis $(i)$ Cloning vector $(b)$ Thermus aquaticus $(ii)$ Construction of first rDNA molecule $(c)$ Agrobacterium tumefaciens $(iii)$ $DNA$ polymerase $(d)$ Salmonella typhimurium $(iv)$ Cry proteins
$(a)\quad (b)\quad (c)\quad (d)$
| $(a)$ Bacillus thuringiensis | $(i)$ Cloning vector |
| $(b)$ Thermus aquaticus | $(ii)$ Construction of first rDNA molecule |
| $(c)$ Agrobacterium tumefaciens | $(iii)$ $DNA$ polymerase |
| $(d)$ Salmonella typhimurium | $(iv)$ Cry proteins |
$(a)\quad (b)\quad (c)\quad (d)$
MediumNEET 2020
View SolutionIdentify $X$ and $Y$ from the given figure of the cloning vector $pBR322$.
Difficult
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