Identify X and Y from the given figure of the | vedclass

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(A) The cloning vector $pBR322$ is a widely used plasmid in biotechnology. In the standard map of $pBR322$,the restriction site for $BamHI$ is located

Vedclass · Accepted Answer

$X = BamHI, Y = PstI$

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(A) The cloning vector $pBR322$ is a widely used plasmid in biotechnology. In the standard map of $pBR322$,the restriction site for $BamHI$ is located within the $tet^R$ (tetracycline resistance) gene,and the restriction site for $PstI$ is located within the $amp^R$ (ampicillin resistance) gene. Based on the standard diagram of $pBR322$,the label $X$ points to the $BamHI$ restriction site,and the label $Y$ points to the $PstI$ restriction site. Therefore,the correct identification is $X = BamHI$ and $Y = PstI$.

Column-$I$	Column-$II$
$(A)$ $\text{YAC}$	$(I)$ Separation of $\text{DNA}$ fragments
$(B)$ $\text{Hind II}$	$(II)$ Yeast
$(C)$ Gel electrophoresis	$(III)$ First restriction endonuclease
$(D)$ Heterologous host	$(IV)$ Recombinant protein

Identify $X$ and $Y$ from the given figure of the cloning vector $pBR322$.

Similar Questions

Match the following:-

Column-$I$ Column-$II$

$(A)$ $\text{YAC}$ $(I)$ Separation of $\text{DNA}$ fragments

$(B)$ $\text{Hind II}$ $(II)$ Yeast

$(C)$ Gel electrophoresis $(III)$ First restriction endonuclease

$(D)$ Heterologous host $(IV)$ Recombinant protein

What is the primary requirement for antibiotic production in biotechnology?

$A$: $cDNA$ libraries are important to scientists in human genomics.
$R$: $cDNA$ is a synthetic type of $DNA$ generated from $mRNA$.

$DNA$ recombinant technology can be:

$Escherichia$ $coli$ $(E. coli)$ is widely used in biological research because:

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