Each restriction endonuclease functions by in | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(C) Restriction endonucleases are enzymes that act as molecular scissors.They recognize specific palindromic nucleotide sequences in $DNA$.Upon recogn

Vedclass · Accepted Answer

Each of the two strands of the double helix at specific points in their sugar-phosphate backbones

Vedclass · Answer

(C) Restriction endonucleases are enzymes that act as molecular scissors.They recognize specific palindromic nucleotide sequences in $DNA$.Upon recognizing the specific sequence,they cleave the phosphodiester bonds in the sugar-phosphate backbone of both strands of the $DNA$ double helix.This cleavage often results in the formation of single-stranded overhangs known as sticky ends or blunt ends,depending on the enzyme used.

Column-$I$	Column-$II$
$(a)$ Cloning vector	$(i)$ Agrobacterium
$(b)$ Ti-$DNA$	$(ii)$ pBR$322$
$(c)$ Disarmed pathogen	$(iii)$ $GAATTC$
$(d)$ Recognition sequence	$(iv)$ Transformation

Each restriction endonuclease functions by inspecting the length of a $DNA$ sequence. It cleaves $ . . . . $

Similar Questions

Match Column-$I$ with Column-$II$ and choose the correct combination from the options given below:

Column-$I$ Column-$II$

$(a)$ Cloning vector $(i)$ Agrobacterium

$(b)$ Ti-$DNA$ $(ii)$ pBR$322$

$(c)$ Disarmed pathogen $(iii)$ $GAATTC$

$(d)$ Recognition sequence $(iv)$ Transformation

The $rop$ gene,which codes for the proteins involved in the replication of the plasmid $pBR322$ in $E. coli$,is located at the restriction site of:

What is required for the functioning of a cloning vector $DNA$?

The term 'disarmed' in a disarmed vector represents:

$A$ : Ethidium bromide helps in visualizing $DNA$ in $UV$ light only.
$R$ : This intercalating agent absorbs light in range $260-330 \; nm$.

Vedclass Test Series

Exam Paper Generator

Online Exam Module