From the following tools/techniques of genetic engineering,identify those which are required for cloning a bacterial gene in animal cells and choose the correct option:
$I$. Endonuclease $II$. Ligase
$III$. $A. tumefaciens$ $IV$. Microinjection
$V$. Gene gun $VI$. Lysozyme
$VII$. Cellulase $VIII$. Electrophoresis

  • A
    $II, III, IV, VII, VIII$
  • B
    $II, III, V, VIII$
  • C
    $I, II, IV, VIII$
  • D
    $I, III, IV, V, VII$

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Identify the correct sequence of steps in the Polymerase Chain Reaction $(PCR)$.

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$R$: Deoxyribonuclease will digest $DNA$ while $RNA$ will be intact.

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Select the correct sequence of steps in $PCR$.

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