Differentiate between $C_3$ and $C_4$ plants.

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(A)
Characteristics$C_3$ Plants$C_4$ Plants
$(1)$ Cell type for Calvin cycleMesophyllBundle sheath
$(2)$ Cell type for initial carboxylationMesophyllMesophyll
$(3)$ Number of cell types fixing $CO_2$MesophyllMesophyll and Bundle sheath
$(4)$ Primary $CO_2$ acceptor$RuBP$$PEP$
$(5)$ Carbon number in primary $CO_2$ acceptor$5C$$3C$
$(6)$ Primary $CO_2$ fixation product$PGA$$OAA$
$(7)$ Carbon number in primary $CO_2$ fixation product$3C$$4C$
$(8)$ Presence of RuBisCoYesYes
$(9)$ Presence of $PEP$ CaseNoYes
$(10)$ Cells containing RuBisCoMesophyllBundle sheath
$(11)$ Photorespiration at low lightNegligibleAbsent
$(12)$ Photorespiration at high lightPresentAbsent
$(13)$ Temperature optimum$20^{\circ}C - 25^{\circ}C$$30^{\circ}C - 45^{\circ}C$
$(14)$ ExamplesWheat,RiceMaize,Sorghum,Sugarcane

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$I.$ Initial $CO_{2}$ acceptor
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$IV.$ Presence of Calvin cycle
$V.$ Leaf anatomy
Which one does not differ in a $C_{3}$ and $C_{4}$ plant?

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