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(B) The production of insulin using recombinant $DNA$ technology was achieved by the American company Eli Lilly in $1983$.They synthesized two $DNA$ s

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Disulphide bond

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(B) The production of insulin using recombinant $DNA$ technology was achieved by the American company Eli Lilly in $1983$.They synthesized two $DNA$ sequences corresponding to the $A$ and $B$-chains of human insulin and introduced them into the plasmids of $Escherichia$ $coli$ ($E$. $coli$) to produce the respective chains.The $A$ and $B$ chains were produced separately,extracted from the bacterial cells,and then combined by creating disulphide bonds to form functional human insulin.

In $1983$,Eli Lilly,an American company,first prepared two $DNA$ sequences corresponding to $A$ and $B$-chains of human insulin and introduced them into the plasmids of $Escherichia$ $coli$ to produce insulin chains. Chains $A$ and $B$ were prepared separately,extracted,and combined by creating:

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