In agarose gel electrophoresis,DNA molecules | vedclass

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(B) In agarose gel electrophoresis,$DNA$ fragments are separated based on their size. Since $DNA$ molecules are negatively charged,they move towards t

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Size only

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(B) In agarose gel electrophoresis,$DNA$ fragments are separated based on their size. Since $DNA$ molecules are negatively charged,they move towards the anode under an electric field. The agarose gel acts as a molecular sieve. Larger $DNA$ fragments encounter more resistance from the gel matrix and move slowly,whereas smaller $DNA$ fragments move through the pores more easily and travel faster. Therefore,the separation is primarily determined by the size of the $DNA$ molecules.

In agarose gel electrophoresis,$DNA$ molecules of different lengths are separated on the basis of their

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