Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.

(N/A) vector is designed to have a unique recognition site for a specific restriction enzyme within its cloning site. If a restriction enzyme has multiple recognition sites within the vector,the vector $DNA$ will be cleaved into multiple fragments upon treatment with that enzyme. This would disrupt the integrity of the vector,making it impossible to insert the gene of interest and successfully create a recombinant $DNA$ molecule.