Main steps in the formation of Recombinant $DNA$ are given below. Arrange these steps in a correct sequence.
$A$. Insertion of recombinant $DNA$ into the host cell
$B$. Cutting of $DNA$ at specific location by restriction enzyme
$C$. Isolation of desired $DNA$ fragment
$D$. Amplification of gene of interest using $PCR$
Choose the correct answer from the options given below:

  • A
    $B, D, A, C$
  • B
    $B, C, D, A$
  • C
    $C, A, B, D$
  • D
    $C, B, D, A$

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Similar Questions

Diagrammatically represent the experimental steps in cloning and expressing a human gene (e.g.,the gene for growth hormone) in a bacterium like $E. coli$.

Given below are two statements.
Statement $I$ - The isolated $DNA$ of donor organism is cleaved by suitable restriction endonuclease to form $DNA$ fragments with cohesive staggered or blunt ends.
Statement $II$ - The foreign $DNA$ or passenger $DNA$ can also be obtained directly from genomic or cDNA library.
In the light of above statements,select the correct option given below:

The steps involved in the Southern blot test are as follows:
$I.$ $X-ray$ film
$II.$ Electrophoresis
$III.$ Digestion with restriction enzyme
$IV.$ Ethidium bromide
$V.$ Radioactive probe
Choose the option having the correct sequential order of these events.

On what basis are $DNA$ fragments separated in agarose gel electrophoresis?

Select the incorrect statement regarding the continuous culture system.

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