Replication was allowed to take place in the | vedclass

Name: Exam Paper Generator | JEE NEET Paper Generator | Vedclass
Brand: Vedclass
Rating: 5 (35 reviews)

Question

(D) In $E. coli$ mutants lacking $DNA$ ligase,the joining of $Okazaki$ fragments on the lagging strand is impaired. During $DNA$ replication,the leadi

Vedclass · Accepted Answer

Graph showing a single peak at low molecular weight.

Vedclass · Answer

(D) In $E. coli$ mutants lacking $DNA$ ligase,the joining of $Okazaki$ fragments on the lagging strand is impaired. During $DNA$ replication,the leading strand is synthesized continuously,while the lagging strand is synthesized as short $Okazaki$ fragments. Normally,$DNA$ ligase joins these fragments into a long,high-molecular-weight strand. In the absence of functional $DNA$ ligase,the newly synthesized $DNA$ remains as short,low-molecular-weight fragments. When these strands are separated by denaturation and subjected to density gradient centrifugation,the short fragments will migrate to the region corresponding to low molecular weight. Therefore,the radioactive signal will show a peak at the low molecular weight position,as represented in graph $(d)$.

Similar Questions

Unwinding of $DNA$ creates tension which is released by enzyme:

$A$: Kornberg enzyme is associated with the removal of primers and thymine dimers.
$R$: $DNA$ polymerase $I$ performs exonuclease activity in $5^{\prime} \rightarrow 3^{\prime}$ and $3^{\prime} \rightarrow 5^{\prime}$ directions.

At what time intervals did Meselson and Stahl take samples after transferring the bacteria to a $^{14}NH_{4}Cl$ medium?

What is an Okazaki segment?

For how long should bacteria be cultured in a $^{14}N$ medium after being transferred from a $^{15}N$ medium so that the extracted $DNA$ consists of equal amounts of hybrid $DNA$ and light $DNA$?

Vedclass Test Series

Exam Paper Generator

Online Exam Module