The significance of the heat shock method in bacterial transformation is that it facilitates:

  • A
    Binding of $DNA$ to the cell wall
  • B
    Uptake of $DNA$ through membrane transport proteins
  • C
    Uptake of $DNA$ through transient pores in the bacterial cell wall
  • D
    Expression of antibiotic resistance gene

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When was the first extra $DNA$ fragment successfully inoculated into $E. coli$ using a plasmid?

The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate.
Given below are two statements about this method :
Statement-$I$ : The blue coloured colonies have $\text{DNA}$ insert in the plasmid and they are identified as recombinant colonies.
Statement-$II$ : The colonies without blue colour have $\text{DNA}$ insert in the plasmid and are identified as recombinant colonies.
In the light of the above statements,choose the most appropriate answer from the options given below:

The components of a bioreactor are:
$I.$ An agitator system
$II.$ An oxygen delivery system
$III.$ Foam control system
$IV.$ Temperature control system
$V.$ $pH$ control system
$VI.$ Sampling ports to withdraw cultures periodically
Choose the correct option.

$DNA$ precipitation out of a mixture of biomolecules can be achieved by treatment with

Which of the following cannot be used for direct gene transfer?

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