Significance of treating bacterial cells with | vedclass

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(C) Since $DNA$ is a hydrophilic molecule,it cannot pass through cell membranes. In order to force bacteria to take up the plasmid,the bacterial cells

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Uptake of $DNA$ by creating transient pores in the bacterial cell wall

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(C) Since $DNA$ is a hydrophilic molecule,it cannot pass through cell membranes. In order to force bacteria to take up the plasmid,the bacterial cells must first be made 'competent' to take up $DNA$. This is done by treating them with a specific concentration of a divalent cation,such as calcium $(Ca^{2+})$,which increases the efficiency with which $DNA$ enters the bacterium through pores in its cell wall. Calcium chloride treatment helps in the binding of $DNA$ to the cell surface and facilitates its uptake by creating transient pores in the bacterial cell wall.

Significance of treating bacterial cells with calcium chloride before transformation is to facilitate

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