The correct order of steps in Polymerase Chai | vedclass

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(A) The Polymerase Chain Reaction $(PCR)$ is a technique used to amplify a specific $DNA$ segment. It consists of three main steps performed in a cycl

Vedclass · Accepted Answer

Denaturation,Annealing,Extension

Vedclass · Answer

(A) The Polymerase Chain Reaction $(PCR)$ is a technique used to amplify a specific $DNA$ segment. It consists of three main steps performed in a cyclic manner: $1$. Denaturation: The double-stranded $DNA$ is heated to a high temperature (usually around $94-98^{\circ}C$) to separate the two strands. $2$. Annealing: The temperature is lowered (usually $50-65^{\circ}C$) to allow the primers to bind to the complementary sequences on the single-stranded $DNA$ templates. $3$. Extension: The temperature is adjusted (usually $72^{\circ}C$) for the $DNA$ polymerase enzyme to synthesize the new $DNA$ strand by adding nucleotides to the primers. Therefore,the correct order is Denaturation,Annealing,and Extension.

The correct order of steps in Polymerase Chain Reaction $(PCR)$ is

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