Which of the following are fused in somatic hybridization involving two varieties of plants?

  • A
    Somatic embryos
  • B
    Protoplasts
  • C
    Pollens
  • D
    Callus

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In tissue culture,a single germinating pollen grain will form a ........ plant.

Hardening in tissue culture is

Assertion $A$: In the plant tissue culture method,the nutrient content of the medium decreases.
Reason $R$: In this method,the biomass of cells or tissues increases.
Which option is correct for Assertion $A$ and Reason $R$?

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