Which of the following is the correct sequenc | vedclass

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(D) The Polymerase Chain Reaction $(PCR)$ consists of three main steps that are repeated in cycles:$1$. Denaturation: The double-stranded $DNA$ is hea

Vedclass · Accepted Answer

Denaturation $\rightarrow$ Annealing $\rightarrow$ Elongation

Vedclass · Answer

(D) The Polymerase Chain Reaction $(PCR)$ consists of three main steps that are repeated in cycles:$1$. Denaturation: The double-stranded $DNA$ is heated to a high temperature (around $94-95^{\circ}C$) to separate the two strands.$2$. Annealing: The temperature is lowered (usually $50-65^{\circ}C$) to allow the primers to bind to the complementary sequences on the single-stranded $DNA$ templates.$3$. Elongation (Extension): The temperature is adjusted (usually $72^{\circ}C$) for the $DNA$ polymerase enzyme to synthesize the new $DNA$ strand by adding nucleotides.Therefore,the correct sequence is Denaturation $\rightarrow$ Annealing $\rightarrow$ Elongation.

List $I$	List $II$
a) Visualization of $DNA$ in gel electrophoresis	i) Chitinase
b) Precipitation of $DNA$	ii) Lysozyme
c) Breaking of cell wall of bacteria	iii) Chilled ethanol
d) Breaking of cell wall of fungus	iv) Ethidium bromide

Which of the following is the correct sequence for $PCR$?

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Match List $I$ with List $II$:
List $I$ List $II$
a) Visualization of $DNA$ in gel electrophoresis i) Chitinase
b) Precipitation of $DNA$ ii) Lysozyme
c) Breaking of cell wall of bacteria iii) Chilled ethanol
d) Breaking of cell wall of fungus iv) Ethidium bromide

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