Which of the following methods is used for the isolation of $DNA$?

Rapid antigen test and $RT$-$PCR$ are two diagnostic tests for the $COVID-19$ virus. $PCR$,a molecular diagnostic tool,stands for:

In the case of polymerase chain reaction $(PCR)$,what are the temperatures required for the following steps?
$A.$ Denaturation
$B.$ Annealing
$C.$ Extension

$I.$ In the process of recombinant $DNA$ technology,after several treatments,the purified $DNA$ is precipitated by adding chilled ethanol.
$II.$ The bacterial,plant,or animal cell is broken down by enzymes to release $DNA$,along with $RNA$,proteins,polysaccharides,and lipids.
Choose the correct option for the above statements.

At which stage of $PCR$,does the template join with the primer?

In which of the following methods is a probe allowed to hybridize to its complementary $DNA$ in a clone of cells?