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(D) The activity of an enzyme is sensitive to various physical and chemical factors. $1$. $Temperature$: Enzymes show highest activity at an optimum t

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Water

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(D) The activity of an enzyme is sensitive to various physical and chemical factors. $1$. $Temperature$: Enzymes show highest activity at an optimum temperature. $2$. $pH$: Enzymes show highest activity at an optimum $pH$. $3$. $Concentration  of  substrate$: The velocity of enzymatic reaction increases with an increase in substrate concentration up to a saturation point. $4$. $Water$: While water is essential for life, it is not considered a direct regulatory factor for enzyme activity in the same manner as temperature, $pH$, or substrate concentration. Therefore, $Water$ is the odd one out.

Delete the odd one out among the factors affecting enzyme activity.

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