The prerequisites for the biotechnological production of antibiotics are:

The correct order of steps in Polymerase Chain Reaction $(PCR)$ is

$PCR$ is used for

Select the option that represents the correct sequence of steps in $r-DNA$ technology.
$(1)$ Insertion of $r-DNA$ into the host
$(2)$ Isolation of $DNA$
$(3)$ Ligation of $DNA$ fragment into the vector
$(4)$ Culturing the host cells in a medium at a large scale and extraction of the desired product
$(5)$ Isolation of the desired $DNA$ fragment
$(6)$ Fragmentation of $DNA$ by restriction endonucleases

Discuss briefly how a probe is used in molecular diagnostics.

What is used for the large-scale production of products in biotechnology?