Which one of the following statements is not true regarding the gel electrophoresis technique?

Consider the following statements:
$I.$ In microinjection method,foreign $DNA$ is directly injected into the nucleus of an animal cell or plant cell by using microneedles or micropipettes.
$II.$ Microinjection method is used in oocytes,eggs,and embryos.
$III.$ Electroporation is the formation of temporary pores in the plasma membrane of a host cell by using lysozyme or calcium chloride.
$IV.$ In chemical-mediated gene transfer method,certain chemicals such as $CO_{2}$ help foreign $DNA$ to enter the host cell.
Which of the statements given above are correct?

Production of a human protein in bacteria by genetic engineering is possible because

Besides better aeration and mixing properties,what other advantages do stirred tank bioreactors have over shake flasks?

The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate.
Given below are two statements about this method :
Statement-$I$ : The blue coloured colonies have $\text{DNA}$ insert in the plasmid and they are identified as recombinant colonies.
Statement-$II$ : The colonies without blue colour have $\text{DNA}$ insert in the plasmid and are identified as recombinant colonies.
In the light of the above statements,choose the most appropriate answer from the options given below:

Which of the following steps is $NOT$ a part of the basic process of creating a Genetically Modified Organism $(GMO)$?