Plasmid $pBR322$ has a $PstI$ restriction enzyme site within the gene $amp^R$ that confers ampicillin resistance. If this enzyme is used for inserting a gene for $\beta$-galactosidase production and the recombinant plasmid is inserted into an $E. coli$ strain:

  • A
    it will not be able to confer ampicillin resistance to the host cell.
  • B
    the transformed cells will have the ability to resist ampicillin as well as produce $\beta$-galactosidase.
  • C
    it will lead to lysis of the host cell.
  • D
    it will be able to produce a novel protein with dual ability.

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